![]() ![]() The operation of the apparatus is monitored by an automated array of pH and ultraviolet absorption sensors under control of a desk-top computer. In the ground-based prototype, laminar flow is provided by a series of parallel filter elements. A new apparatus for large scale IEF, utilizing a recycling principle, has been developed. Isoelectric focusing ( IEF) is a powerful variant of electrophoresis, in which amphoteric substances are separated in a pH gradient according to their isoelectric points. The potential of space electrophoresis is conditioned by the fact that all electrophoretic techniques require the suppression of gravity-caused convection. ![]() Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-based signal amplification of ELISA and the concentration of enzyme reaction products by c IEF.īier, M. This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-c IEF). Henares, Terence G Uenoyama, Yuta Nogawa, Yuto Ikegami, Ken Citterio, Daniel Suzuki, Koji Funano, Shun-ichi Sueyoshi, Kenji Endo, Tatsuro Hisamoto, Hideaki Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-c IEF). ![]()
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